The effect of muscle length on transcranial magnetic stimulation‐induced relaxation rate in the plantar flexors

Transcranial magnetic stimulation (TMS) of the motor cortex during a maximal voluntary contraction (MVC) permits functionally relevant measurements of muscle group relaxation rate (i.e., when muscles are actively contracting under voluntary control). This study's purpose was twofold: (1) to explore the impact of muscle length on TMS-induced plantar flexor relaxation rate; and (2) to incorporate ultrasonography to measure relaxation-induced lengthening of medial gastrocnemius (MG) fascicles and displacement of the muscle-tendon junction (MTJ). Eleven males (24.8 ± 7.0 years) performed 21 brief isometric plantar flexor MVCs. Trials were block-randomized every three MVCs among 20° dorsiflexion (DF), a neutral ankle position, and 30° plantar flexion (PF). During each MVC, TMS was delivered and ultrasound video recordings captured MG fascicles or MTJ length changes. Peak relaxation rate was calculated as the steepest slope of the TMS-induced drop in plantar flexor torque or the rate of length change for MG fascicles and MTJ Torque relaxation rate was slower for PF (-804 ± 162 Nm·s-1) than neutral and DF (-1896 ± 298 and -2008 ± 692 Nm·s-1, respectively). Similarly, MG fascicle relaxation rate was slower for PF (-2.80 ± 1.10 cm·s-1) than neutral and DF (-5.35 ± 1.10 and -4.81 ± 1.87 cm·s-1, respectively). MTJ displacement rate showed a similar trend (P = 0.06), with 3.89 ± 1.93 cm·s-1 for PF compared to rates of 6.87 ± 1.55 and 6.36 ± 2.97 cm·s-1 for neutral and DF, respectively. These findings indicate muscle length affects the torque relaxation rate recorded after TMS during an MVC Comparable results were obtained from muscle fascicles, indicating ultrasound imaging is suitable for measuring evoked contractile properties during voluntary contraction.